منابع مشابه
Re-visiting the trans insertion model for complexin clamping
We have previously proposed that complexin cross-links multiple pre-fusion SNARE complexes via a trans interaction to function as a clamp on SNARE-mediated neurotransmitter release. A recent NMR study was unable to detect the trans clamping interaction of complexin and therefore questioned the previous interpretation of the fluorescence resonance energy transfer and isothermal titration calorim...
متن کاملGenetic analysis of the Complexin trans-clamping model for cross-linking SNARE complexes in vivo.
Complexin (Cpx) is a SNARE-binding protein that regulates neurotransmission by clamping spontaneous synaptic vesicle fusion in the absence of Ca(2+) influx while promoting evoked release in response to an action potential. Previous studies indicated Cpx may cross-link multiple SNARE complexes via a trans interaction to function as a fusion clamp. During Ca(2+) influx, Cpx is predicted to underg...
متن کاملComplexin 2
Complexins are a small family of proteins comprised of 4 members – complexin 1-4. Complexin 1, originally identified as synaphin, is a small 134 amino acid cytosolic protein that is expressed exclusively in neurons where it associates with soluble N-ethylmaleimidesensitive factor attachment protein receptor (SNARE) complexes necessary for exocytosis (16,25). Complexin 2 is ubiquitously expresse...
متن کاملC-terminal complexin sequence is selectively required for clamping and priming but not for Ca2+ triggering of synaptic exocytosis.
Complexins are small soluble proteins that bind to assembling SNARE complexes during synaptic vesicle exocytosis, which in turn mediates neurotransmitter release. Complexins are required for clamping of spontaneous "mini " release and for the priming and synaptotagmin-dependent Ca(2+) triggering of evoked release. Mammalian genomes encode four complexins that are composed of an N-terminal unstr...
متن کاملPCR Clamping 27 PCR Clamping
An efficient, PCR based method for the selective amplification of DNA target sequences that differs by a single base pair is described. The method utilises the high affinity and specificity of PNA for their complementary nucleic acids and that PNA cannot function as primers for DNA polymerases.
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ژورنال
عنوان ژورنال: Nature Reviews Molecular Cell Biology
سال: 2009
ISSN: 1471-0072,1471-0080
DOI: 10.1038/nrm2650